(Box of 25) PG Progesterone Test and Canine PG Chip
25 Test and 1 ID chip per box
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Progesterone test consist of a “test cartridge” and ID chip and a Detection buffer.
The test Cartridge contains a test strip; on the membrane of which, progesterone- BSA conjugate and chicken IGY have been immobilized at the test line and the control line respectively.
Each test cartridge is individually sealed in an aluminum foil pouch containing a desiccant. 25 sealed test cartridges are packed in a box which also contains and ID chip.
The detection buffer pre-dispensed in a tube contains flourochrome-labeled anti-progesterone antibodies, fluorescent-labeled anti-chicken IgY, bovine serum albumin (BSA) as a stabilizer and less than 0.1% sodium azide in phosphate in phosphate buffered saline (PBS) as a preservative.
The Detection buffer is packed in a separate box which is further packed in a Styrofoam box provided with ice packs for the purpose of shipment.
P4 Progesterone Analyzer Progesterone level Chart
Progesterone also known as P4 (Pregn-4-ene-3,20-Dione) is a C-21 Steroid hormone involved and the bitch heat cycle, Pregnancy (supports gestation) and embryo genesis of different species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring progestogen.
In mammals, progesterone, like all other steroid hormones, is synthesized from pregnenolone, which in turn is derived from Cholesterol.
Progesterone is assential for the regulation of the normal females reproductive functions. The Major physiological actions of progesterone are : a) in the uterus and ovary b) in the mammary gland: lubular-alveolar development in preparation for milk secretion; c) in the brain: neurobehavioral expression associated with sexual responsiveness and d) in the bone: prevent of bone loss.
During the follicular phase of the heat cycle, progesterone levels remain low following the LH surge and ovulation, luteal cells in the ruptured follicle produce progesterone in response to LH. During this, the luteal phase, progesterone transforms estrogen-primed endometrium from a proliferative to a secretory state. If conception accurs, the levels of progesterone are maintained at mid-luteal levels by the corpus luteum until about week six. At the time the placenta becomes the main source of progesterone and levels rise from approximately 50-280 ng/mL.
Serum Progesterone Concentrations
Progesterone concentration begins to increase prior to ovulation in the bitch due to luteinization of small follicles on the ovaries. An indicator that ovulation is impending is the progesterone surge. This surge occurs when progesterone concentrations are around 2 ng/ml (or 6.2 nmol/l) or when the baseline progesterone concentration doubles. In many bitches, ovulation will occur 36 – 48 hours after the progesterone surge. Progesterone concentrations rise to between 4 and 10 ng/ml at the time of ovulation (12.6 and 31.4 nmol/l). Before ovulation, progesterone rises slowly, while after ovulation progesterone rises rapidly. A jump of at least 3 – 4 ng/ml (9.14– 12.56 nmol/l) in a 24 hour period, after progesterone concentrations reach 4 ng/ml, is confirmatory of ovulation. Some bitches may jump as much as 8 – 10 ng/ml in a 24 hour period after ovulation has occurred. Some bitches will stall between the progesterone surge and ovulation having values between 2.5 and 4.0 ng/ml (7.8 and 12.5 nmol/l) for several days.
Following ovulation it takes the eggs 2 days to mature and be ready to be fertilized. Ovulation of multiple follicles may occur over a 12 – 36 hour period. Once mature, the eggs are capable of being fertilized for 2 days. Therefore, the fertile period begins 2 days after ovulation and lasts for the next 3 – 4 days. Typically, the first day of breeding (day 4 after the LH surge) will occur when the progesterone concentration is between 12 and 15 ng/ml (37.2 and 46.8 nmol/l). It is recommended that ovulation be confirmed (rather than depending on normal progression of ovulatory events after the progesterone surge) because of the tendency for some bitches to stall between the surge and ovulation or for some bitches to fail to ovulate completely (due to split heats, inadequate hormone production from the brain, or abnormal follicular development). Progesterone testing is generally performed every 2 days, until ovulation is imminent and then daily until ovulation is confirmed.
Recommended operation condition for the Progesterone, Temperature: 20-30 C and 68-89.6 F <70%
1. Transfer 30uL of simple (serum or plasma or control) using a transfer pipette to a tube containing the detection buffer.
2. Close the lid of the detection buffer tube and mix the simple thoroughly by shaking it about 10 times.
3. Pipette out of 75uL of a simple Mixture and dispense it into the simple well on the test cartrirdge.
4. Leave the simple-loaded test cartridge at room temperatura for 15 minutes.
5. For scanning the sample-loader test cartridge, insert it into the test the cartridge holder of your reader, ensure proper orientation of the test cartridge before pushing it all the way inside the test cartridge holder. An arrow has been marked on the test cartridge specially for this purpose.
6. Press “select” on your reader to start the scanning process.
7. The reader will start scanning the sample-loaded test cartridge immediately.
8. Read the result in ng/mL the reader Working range is from 1.4 to 40 ng/ml (4.45-12702 nmol/L). on the display screen of your reader.
This fluorescence scanning instrument will also test Cortisol, Fsh, LH, PRL, T4, Testosterone, TSH
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